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Caspase-4 Fluorometric Assay Kit

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Caspase-4 Fluorometric Assay Kit

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Quick Overview

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (400 nm); upon cleavage of the substrate by caspase-4 or related caspases, free AFC emits a yellow-green fluorescence (505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.

Product Description

Caspase-4 Fluorometric Assay Kit

Additional Information

Catalog Number K126-25
Specificity No
Application No
Measuring Range No
Sensitivity No
Format 25 assays

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