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Caspase-3 Colorimetric Assay Kit

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Caspase-3 Colorimetric Assay Kit

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Quick Overview

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

Product Description

Caspase-3 Colorimetric Assay Kit

Additional Information

Catalog Number K106-25
Specificity No
Application No
Measuring Range No
Sensitivity No
Format 25 assays

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